ViroKey SQ FLEX SARS-CoV-2 Genotyping Assay (RUO)

Detection for mutation, through whole genome sequencing of SARS-CoV-2


The ViroKey® SQ FLEX SARS-CoV-2 Genotyping Assay is a next-generation sequencing-based test intended for the detection of genomic mutations of the SARS-CoV-2 RNA genome from nasopharyngeal or oropharyngeal swab samples to support molecular epidemiology of SARS-CoV-2 research.

The  ViroKey SQ FLEX SARS-CoV-2 Genotyping Assay is used in conjunction with the Sentosa® SX Virus Total Nucleic Acid Plus (4x24) as well as the Sentosa SQ Virus Testing Solutions on the Sentosa SX101, Sentosa ST401 and Sentosa SQ301 instruments.


High Performance and Robustness:

  • > 99% coverage of the SARS-CoV-2 genome
  • ≥ 200x depth of coverage

Providing Laboratory Efficiency and Effectiveness:

  • Able to extract up to 15 samples (plus controls) per run
  • Reliable and reproducible variant calling
  • Automated workflow, from samples to results in 2 days
  • Minimal hands-on time of < 2 hours
  • Automatic bioinfomatics analysis and report generation (including PANGO Lineage and Nextclade Analysis) with Sentosa SQ Reporter



Parameters Specification
Sample Types

Nasopharyngeal and Oropharyngeal Swabs in UTM/VTM or PBS/Liquid Amies/Saline

Sample Input

730 μL 

Sample Throughput

Up to 15 samples per run 


System Control, Extraction Control, Positive Control (Optional) 


≥ 200X 

Limit of Detection (LoD)

1000 GE/mL 

Hands-on Time

< 2 hours 

Turnaround Time

~2 days (from sample to results) 



ProductPack SizeItem Number
Sentosa SX Virus Total Nucleic Acid Plus Kit (4x24) CE-IVD 4x24 300154
Sentosa SX Virus Total Nucleic Acid Plus Kit (4x24) RUO 4x24 300155
Sentosa SX Virus Total Nucleic Acid Plus Kit (4x24) US-IVD 4x24 300395
ViroKey SQ FLEX Library Prep Reagents (4x16) RUO 4x16 300823
ViroKey SQ FLEX SARS-CoV-2 Primer Set (96) RUO 96 301091
ViroKey SQ SARS-CoV-2 Positive Control Kit (8) RUO 8 301092