Sentosa SA Direct MRSA/SA PCR Test

Fast detection and differentiation of MRSA targets


The Sentosa® SA Direct MRSA/SA PCR Test is a real-time polymerase chain reaction (PCR)-based test for the qualitative detection and differentiation of Staphylococcus aureus (SA) and methicillin-resistant Staphylococcus aureus (MRSA) from nasal swabs.

The test is validated for use with the Sentosa SX Lysis Beads, followed by manual PCR setup, in conjunction with PCR on the Rotor-Gene Q MDx 5plex HRM instrument and automated result calling and report generation with Sentosa SA Reporter.

For Research Use Only. Not for use in diagnostic procedures.


Supporting Decision Making

  • Sensitive and robust
  • Differentially detects MRSA and SA
  • Detects type I to VII and XI SCCmec/orfX cassettes
  • Detects new MRSA strain mecC

Providing Laboratory Efficiency

  • Very short turn-around time: 2 hrs 26 mins for 24 samples 
  • Only 40 mins of hands-on work
  • Automatic result call-out within 2 mins
  • Tests up to 240 reactions per day

Increasing Cost Effectiveness

  • Three targets in one test to save reagents and consumables
  • Flexibility to test 1 – 48 reactions/run
  • Comprehensive and fast result call-out to reduce patient management cost



Analytical Sensitivity

SA: 50 CFU/swab (95% CI: 20.02 – 124.89 CFU/swab)

MRSA: 200 CFU/swab (95% CI: 84.59 – 472.85 CFU/swab)

Analytical Reactivity

9 SCCmec types (I, II, III, IV, V, VI, VII, VIII and XI)

11 PFGE types (USA100, USA200, USA300, USA400, USA500, USA600, USA700, USA800, USA900, USA1000 and USA1200)
Analytical Specificity No cross-reactivity with 138 other microorganisms tested

99.77% (95% CI: 98.70 - 99.96%)

Sample Type(s) Nasal swabs


Sentosa SA Direct MRSA/SA PCR Test Flyer RUO EN


ProductPack SizeItem Number
Sentosa SA Direct MRSA/SA PCR Test (4x24) RUO 4x24 300244
Sentosa SX Lysis Beads (96) RUO 96 tubes 300141
Sentosa SX Lysis Plate (10x96) RUO 10x96 300705
Sentosa SX Lysis Plate (96) RUO 96 300650