PathoKey® SQ FLEX Pathogen ID Assay
Qualitative Detection of Pathogens from Complex Microbiome Samples
The PathoKey SQ FLEX Pathogen ID Assay is a next-generation sequencing (NGS)-based test that provides comprehensive and robust profiling of bacterial, fungal, eukaryotic communities and viruses from sources such as gut microbiome, contaminated food and clinical samples respectively.
It has the flexibility to interrogate any combination of bacterial 16S hypervariable regions (V1-V2 and V3-V5), and rpoB regions, fungal hypervariable regions (ITS1 and ITS2), and 28S region (D2), and eukaryotic community 18S hypervariable regions (V3 and V8-V9), and 28S region (D2). In addition, the assay detects up to 10 viruses and 32 AMR genes.
This test is intended to be used in conjunction with the Sentosa® SX Lysis Plate Type B and Sentosa SX U-Prep Kit on the Sentosa SX101 instrument, Ion PGM Hi-Q View OT2 Kit and Sentosa ST Template Beads on the Sentosa ST401 instrument, Ion PGM Hi-Q View Sequencing Kit (with Ion PGM Wash 2 Bottle Kit) and Sentosa SQ 318 Chip Kit (8) on the Sentosa SQ301 instrument as well as automated data analysis and report generation on the Sentosa SQ Reporter software.
Unparalleled Versatility
- Five panels to choose from: bacteria, fungi, parasites, viruses and genotypic antimicrobial resistance (AMR)
- Amplicon sequencing allows for identification of up to four groups in one run
Comprehensive Detection
- Compares all detected pathogens to a database of >500,000 known sequences
- Relative abundances are reported, allowing for differentiation of commensals or environmental contamination vs pathogenic microbes
Maximizing Laboratory Efficiency
- No need to spend extended periods of time culturing samples
- Sample-to-results in two days, with less than two hours of hands-on-time
- Highly automated NGS workflow that is easy to adopt, with automated data analysis and report generation with Sentosa SQ Reporter
| Sample Types* | Serum, stool, nasopharyngeal swabs, skin, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), plasma, whole blood, formalin-fixed paraffin-embedded (FFPE) tissue, saliva, urine | |||||||||||
| Sample Throughput | 22 samples per run | |||||||||||
| Turnaround Time | Approximately two days (from sample to results) | |||||||||||
| Hands-On Time | 65-100 minutes | |||||||||||
| Controls | One positive control, One negative control, One extraction control | |||||||||||
| Targets |
Bacteria: V1-V2 and V3-V5 hypervariable region in 16S, and rpoB gene Fungi: ITS1 and ITS2 hypervariable region, and D2 region in 28S Eukaryotes: V3 and V8-V9 hypervariable region in 18S, and D2 region in 28S Viruses: 10 types of viruses AMR: 32 AMR genes |
|||||||||||
| Number of Detectable Pathogens |
>2000 |
|||||||||||
*Some sample types may require pre-treatment.
| PathoKey SQ FLEX Pathogen ID Assay Flyer | RUO | EN |
| Product | Pack Size | Item Number |
|---|---|---|
| Sentosa SQ Virus Testing Solutions (4x24) RUO | 4x24 | 300775 |
| PathoKey SQ FLEX AMR ID Kit (96) RUO | 4x24 | 301083 |
| PathoKey SQ FLEX Bacteria ID Kit (96) RUO | 4x24 | 301079 |
| PathoKey SQ FLEX Fungi ID Kit (96) RUO | 4x24 | 301080 |
| PathoKey SQ FLEX Library Prep Reagents (4x24) RUO | 4x24 | 301078 |
| PathoKey SQ FLEX Parasite ID Kit (96) RUO | 4x24 | 301081 |
| PathoKey SQ FLEX Pathogen Positive Control (4) RUO | 4 | 301084 |
| PathoKey SQ FLEX Virus Panel I ID Kit (96) RUO | 4x24 | 301082 |
| Sentosa SQ Reporter v2.5 | 480204 | |
| Sentosa SX Lysis Plate Type B (10x24) RUO | 10x24 | 300792 |
| Sentosa SX U-Prep Kit (4) RUO | 4 | 300836 |
For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.
For more product related documents, please contact us.
